Fig. 2. Low caveolin-1 protein in fat tissues of SNTB2^-/- mice and upon knock down of SNTB2 in 3T3-L1 cells. A. ATGL, HSL, perilipin, adipophilin and FSP27 in epididymal (epi) fat of wild type (WT) and SNTB2^-/- (B2) animals. B. pACC, FAS, SCD-1, FABP4 and ABCA1 protein in epi fat of these animals. C. SNTB2 and caveolin-1 in subcutaneous (sc), epi and perirenal (ren) fat of WT and SNTB2^-/- animals injected with insulin or water. D. Quantification of caveolin-1 protein in sc and ren fat of 8 WT and 7 SNTB2^-/- animals. E. Caveolin-1, SNTB2 and GAPDH in mature 3T3-L1 adipocytes differentiated from preadipocytes which were transfected with scrambled or SNTB2 siRNA before differentiation. F. Protein densitometry of SNTB2 and caveolin-1 in the cells described in E (n = 4 - 20). G. Caveolin-1, SNTB2 and GAPDH in 3T3-L1 adipocytes transfected with scrambled or SNTB2 siRNA after differentiation. H. Protein densitometry of SNTB2 and caveolin-1 in the cells described in G (n = 7 - 11). I. Analysis of syntrophins (using the pan syntrophin antibody) and caveolin-1 in proteins precipitated by SNTB2 or caveolin-1 antibodies from preadipocyte and adipocyte lysates. J. Analysis of SNTB2 and caveolin-1 protein precipitated from adipocyte lysate using antibodies specific for syntrophins or utrophin. Lysate, L; * p<0.05, ** p<0.01, *** p<0.001.